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Department of Agriculture, Agricultural Research Service.2011

Isolation of adipocyte-rich fraction For adipocyte fractionation, freshly harvested tissue was finely minced with scissors and digested using Collagenase Type II (1 mg/ml, Thermo Fisher Scientific, Waltham, MA, United States) dissolved in PBS (Thermo Fisher Scientific, Waltham, MA, United States), supplemented with 10 mM CaCl2 (Sigma-Aldrich, St