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Description

Funding The study was supported by a grant (19-2215) from the Life Sciences College at Brigham Young University

Bars indicate mean responses in each group, with error bars indicating standard deviations

Temporal and spatial activation of TGF- is essential for tissue homeostasis TGF- proteins belong to the TGF- superfamily, which consists of TGF-13, the activins/inhibins/Mllerian-inhibiting substances (MIS), bone morphogenetic proteins (BMPs), Nodal, growth/differentiation factors (GDFs), and the distantly related glial cell line-derived neurotrophic factors (GDNF) family

For immunoprecipitation, anti-FLAG (Sigma-Aldrich, M2, A2220), anti-HA (Sigma-Aldrich, A2095), and anti-OTUD5 (Cell Signaling Technology, 20087, 1:1,000) antibodies were used