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Chen S, Bao X, Chen H, Jia M, Li W, Zhang L, et al

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Single colonies obtained at the highest possible antibiotic concentration were picked and tested for successful deletion of the target gene by colony PCR using suitable flanking primers, followed by double-stranded DNA sequencing of the amplified region (Eurofins Genomics)

In particular, mast cells signal to microglia, the resident immune cells of the brain, priming them toward a pro-inflammatory phenotype