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In the co-culture system, MCECs derived from the model group exhibited significant reduced ZO-1 and Occludin expression compared to those from the Control group, whereas GHK-Cu elevated theses protein levels (Figures 4GI)

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Additional modelling of the flexible catalytic domains was done by manually fitting the catalytic domain structure from PDB 4PCU, using ChimeraX 60 , into the 8.3 map of a single catalytic domain

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