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Formaldehyde induces diabetes-associated cognitive impairments

For the measurement of total chlorophyll of leaves, a 0.5 g leaf sample was homogenized in 5 mL acetone (80% v/v), followed by centrifugation at 10,000 g for 8 min, after which the optical density was measured at 663 and 653 nm for Chl a and Chl b , respectively, using a spectrophotometer (Beckman 640 D, USA) by following the method of Arnon 34

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A second undiluted injection of liquid-form PCL (volume: 1 cc, delivered via a 23 G 50 mm cannula) was administered to the middle part of the face region on October 6, 2023

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