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J Pathol (2017) 241(4):53446

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Samples were resuspended in 0.1% trifluoroacetic acid (TFA) and desalted by reversed-phase HPLC on a Phenomenex Jupiter C4 column (250 mm 2.0 mm, 300 pore size) with a linear gradient from 10% to 95% of solvent B (0.07% TFA in 95% acetonitrile) in 30 min, at a flow rate of 200 L/min using an Agilent Technologies 1100 HPLC (Agilent Technologies, USA) 3