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In: Greene CE, editor

10.1016/j.ymthe.2019.04.019 22 CimadomoD.RienziL.ConfortiA.FormanE.CanosaS.InnocentiF.et al (2023)

The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm