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Notably, the aerobic treatments showed significantly higher levels of both markers, indicating enhanced ROS generation under oxygen-rich conditions (Fig

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Using nile red and oil red O staining to detect lipid accumulation, and cytokines bioplex assay to assess inflammation, it was found that olive leaf extracts at concentrations of 25, 50 and 100 g/mL were able to reduce intracellular lipid content and thereby combated the intracellular inflammatory state (Musolino et al., 2022)