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Briefly, HUVECs (1.1105 cells/cm2) were sandwiched between two layers of collagen gel (0.258% type I collagen) with tube-induction medium composed of MCDB-104GK medium and 199 medium at a 13:7 ratio, supplemented with 2% FBS, l-ascorbic acid (25 g/ml), penicillin (5 g/ml), streptomycin (5 g/ml) and neomycin (10 g/ml) in 24-well culture plates (Becton Dickinson Labware) for 24 h

Novel concepts of neuropeptide-based drug therapy: vasoactive intestinal polypeptide and its receptors

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