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Although immortalization and early authentication concerns place some limitations on its translational accuracy, the ATCC-certified HMC3 line represents the most reliable and reproducible platform for routine experimental studies (162)

SIRT1 also indirectly modulates key molecules in the insulin signaling pathway, such as insulin receptor substrate 2 (IRS2) by influencing members of the forkhead transcription factors (FOXO) family, ultimately alleviating insulin resistance [108,109,110]

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