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Multiple studies have demonstrated that consistent administration of HCQ and CQ during the first five years of the disease improves treatment outcomes, whether used as monotherapy or in combination with other therapies (104)

Fluorescence microscopy Microscopy images of nucleosome array condensates were captured with an inverted IX83 Olympus microscope with a 16-channel LED light source, equipped with a temperature-controlled chamber and a sCMOS camera (Hamamtsu Orca Flash4) using an Olympus LUC PLAN FLN 40x LWD/0.60 objective

Thermo Fisher Scientific, AM4302, RRID:AB_2536382), CS (1:1000

Although the precise mechanisms by which BAs modulate immune responses at the maternal-fetal interface remain unclear, emerging clinical evidence links abnormal serum total BA levels to pregnancy complications, including GDM and ICP 289,297,298

However, the role of T cells has been questioned in several studies and further investigations are required to determine the exact mechanisms involved in the pathogenesis of this disorder [2]

however, it is important to be aware of their associated risks