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Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution

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The amount of reported b gas and ME of PC were 35.77 ml/200 mg of DM and 4.12 MJ/kg DM, respectively [139]

Am J Clin Nutr 1994;60:2-11

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