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We used female and male littermate mice that were age-matched between experimental groups

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The central research question this article examines is a mechanistic one: if MGF is a naturally occurring splice product of the IGF-1 gene that acts locally and transiently in mechanically loaded muscle, while IGF-1 LR3 is an engineered analog built to evade the bodys clearance system and act systemically for hours, how do these two IGF-1 variants actually differ at the level of receptor engagement, binding-protein interaction, and satellite-cell biology