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Forming a biofilm is a very effective protective mechanism and bacteria encased in a biofilm are highly resistant to antibiotics (up to 10,000-fold)

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Methods for obtaining heavy chain antibodies and the variable domains are inter alia provided in the following references: WO 94/04678, WO 95/04079, WO 96/34103, WO 94/25591, WO 99/37681, WO 00/40968, WO 00/43507, WO 00/65057, WO 01/40310, WO 01/44301, EP 1134231, WO 02/48193, WO 97/49805, WO 01/21817, WO 03/035694, WO 03/054016, WO 03/055527, WO 03/050531, WO 01/90190, WO 03/025020, WO 04/041867, WO 04/041862, WO04/041865, WO 04/041863, WO 04/062551

American Society for Therapeutic Radiology and Oncology 69(3):S106, 2007

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This transformation is marked by the expression of T-bet and gamma interferon, an increase in PI3K/AKT signaling activity, and a subsequent loss of their suppressive function over effector T cells (142) ( Figure 6 )