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aeruginosa (PAK strain) were cultured overnight, subcultured until midexponential phase then opsonized in human serum (Sigma-Aldrich, no

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CRISPR Cas9 gene editing Target genes were disrupted in MODE-K IECs using the CRISPR/Cas9 system 26

Closely intertwined with endothelial dysfunction is the profound mitochondrial impairment observed in patients with complex chronic illnesses

The oxidative stress in mitochondria could be counteracted by IDH1-driven reductive carboxylation in the cytosol, followed by the transfer of citrate into the mitochondria, which was to be oxidized by IDH2 to generate NADPH [104]

Subsequent analysis and other research 210 demonstrated that cells exhibiting high OCR and increased ROS production were capable of expressing higher levels of ALDH1A1