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We also found both significant association with CARS item4 (bizarre use of body movement and persistence of stereotypes) ( r = 0.37, p = 0.0466, n = 35) and item 5 (peculiarities in relating to non-human objects such as toys and other materials) ( r = 0.47, p = 0.0084, n = 35) (Supplementary Table 2)

Peaks were observed for each of the six sweep backgrounds, and these overlapped genes with products linked to insecticide resistance, including cytochrome P450 4 , glutathione S-transferase 1 , acetylcholinesterase 6 , cytochrome b5 48 , cytochrome c oxidase 49 , esterase B1 50 , Nach sodium channel protein 3 , cholinesterase 51 , UDP-glycosyltransferase 52 , carbonic anhydrase II 53 , trypsin and anti-chymotrypsin 54 , peroxiredoxin 55 , and sodium-coupled monocarboxylate transporter 56

The cluster analysis was performed using R (version 3.6.1) with the hclust package 32

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The area under the curve of receiver operating characteristic curve of sMSAPRP was 0.74 and 0.84 in each group