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FIGURE 1 Generally, HSP70 binding sites in polypeptide chains repeat every 36 residues, mainly in -sheets with four to five residues, such as leucine, isoleucine, valine, phenylalanine, and tyrosine (Rdiger et al., 1997)

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S9-mix consisted of 10% (v/v) non-induced hamster or induced rat liver S9-fraction (1 volume S9) and 9 volumes of the respective co-factors (8 mM MgCl 2 , 33 mM KCl, 5 mM glucose-6-phosphate, 4 mM NADP) in sodium orthophosphate buffer (100 mM, pH 7.4)

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A standard metered-dose pharmaceutical spray actuates approximately 0.1 ml (100 microlitres) per pump, so ten sprays empty one millilitre of solution