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Then, the plant material was transferred into 1% OsO 4 in 0.1 M phosphate-buffered saline solution for 12 h at room temperature, followed by three washes in 0.1 M phosphate-buffered saline solution for 15 min each

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doi: 10.1089/jir.2011.0065 233 McKinneyEFSmithKG

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The reaction mixtures contained 50 mM Tris-HCl buffer (pH 7.5), 1 mM EDTA, 500 M NADPH, 5 1 1mg/ml crude enzyme, the relevant electron donor and substrates