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The variance was filtered by default optimisation settings and a predefined standard deviation of p = 0.005 comparing untreated HepG2 and HepG2 ATP7B-KO resulted in an FDR of q = 0.01788

The peptide GHK spontaneously complexes with copper and facilitates the uptake of copper by cells

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The cell viability was measured by absorbance of all samples at 450 nm in a microplate reader (SpectraMax, Plus 384, Molecular Devices, Inc., USA) under CCK-8 (Cell Counting Kit-8, Dojindo, Kumamoto, Japan) reagent