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In addition, we have performed proof-of-principle experiments that such experimental models will prove useful in the discovery and validation of molecularly targeted therapies

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This effect was associated with increased expression of IGF-1 and VEGF in the primary ossification zone.103 Altogether, the bone anabolic actions of the GH can be, at least in part, mediated by paracrine IGF-1 signaling, which enhances osteoblast activity.104 In fact, the only administration of recombinant IGF-1 to healthy participants results in an increase in circulating levels of bone remodeling markers

3.4.3 Incentive mechanisms for the adaptation of FBS alternatives The reduction and replacement of FBS use with alternatives are becoming feasible as hPL and other serum-free substitutes have demonstrated performance comparable to that of FBS, supporting MSCs proliferation without compromising phenotypic stability, and enhancing monoclonal antibodies production in hybridoma cell lines (Even et al., 2006

doi: 10.1021/acsami.5c12666 99 SarlakN.TaherifarA.SalehiF

It is difficult at this stage to establish a causal sequence of events that underlie these observations