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2.5.3 Determination of KCC2 KCC2 levels were measured using an ELISA kit (ELK Biotechnology, ELK0481, Wuhan, China)

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[1] A relevant honesty note: the antidote for acetaminophen poisoning is N-acetylcysteine (NAC) , which replenishes glutathione by supplying cysteine not glutathione itself underscoring that raising intracellular glutathione is usually achieved through precursors, not direct administration

Altering Ca 2+ balance can regulate activity of key enzymes in de novo lipogenesis, including fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 ( Scd1 ), or, induce lipolysis [12, 13]

The rest is better guided by measurement than by default