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An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm

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Zhao, X., Liu, L., An, T., Xian, M., Luckanagul, J

T., Vinkers, C

Results: 11/15 FMT recipients demonstrated metabolic profile shifts towards those of the healthy donors after receiving three FMTs and were deemed responders

Vit D regulates the epigenetic programming of immune cells, promotes immunological tolerance in T-cells, and reduces the inflammatory response, all of which contribute to MS pathogenesis (77)