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( G ) Depletion of SQR in HeLa cells, as demonstrated by fluorescent antibody labeling

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After transfer of 100 L of the respective cell suspensions into a black, clear-bottom 96-well plate (Nunc, Rochester, NY), 5 mM of the substances of interest: buffer, reduced (Sigma-Aldrich, CAS-70-18-8, GSH) or oxidized (Sigma-Aldrich, CAS-27025-41-8, GSSG) glutathione, cysteine or cystine, -mercapthoethanol or DTT were added