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Interestingly, by comparing ROS expression in both wound healing (early stage, lower levels) and regeneration (later stage, elevated levels) in planarians, Van Huizen et al

This is one reason why researchers interested in microdosing often reconstitute with more water (3mL or even 4mL) to get a lower concentration that makes small doses easier to measure

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doi: 10.1177/03009858211012949

Some researchers include outer thighs and upper arms in their rotation to provide even more sites

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