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Materials and methods Cloning and construction of GST isozymes overexpression plasmids The total mRNA was extracted from the Cobb broiler to generate the cDNA using the reverse transcription kit (Takara Bio Inc., Kusatsu, Japan)

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Vagus nerve assessment via ultrasonography in irritable bowel syndrome

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deacetylation of PGC-1 mitochondrial biogenesis SIRT3 (mitochondrial): deacetylation of mitochondrial enzymes better OXPHOS efficiency SIRT6 : stabilization of telomeres, DNA repairs SIRT2 : regulation of metabolism and the cell cycle Sirtuin activation is the main longevity mechanism associated with NAD+