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Annu Rev Med (2018) 69(1):43749

the antibody constructs of the present invention typically have different isoelectric point (pi) values for their first and second domain

All our vials arrive in the lyophilized (powdered) form and must be reconstituted with bacteriostatic water prior to research use

Acknowledgements Work in the authors laboratories was supported by Ministerio de Ciencia e Innovacin (MCIN), Agencia Estatal de Investigacin (AEI) MCIN/AEI/10.13039/501100011033 grant PID2020-113521RB-I00

The collected sample aliquots were immediately frozen in liquid nitrogen and stored at -80C until further processing

Given the fairly short half-life of unbound IGF-1 in serum (i.e., 510 min), binding to an IGF binding protein (IGFBP-3 is the most prevalent) in serum or in ECM increases IGF-1 half-life to around 25 min (Allard and Duan, 2019), leading to the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) (O'Neill et al., 2015)