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Abstract Background Glutathione (GSH), a pivotal non-protein thiol, can be biosynthesized through three pathways in different organisms: (1) two consecutive enzymatic reactions catalyzed by -glutamylcysteine synthetase (Gsh1 or GshA) and glutathione synthetase (Gsh2 or GshB)

Nat Protoc 1 , 31593165 (2006)

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Metabolite quantification was acquired using Barstool [52] (see Supplementary Fig

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