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Bacteria were grown at 30 C or 37 C, depending on the species and 6 cm nematode growth medium (NGM) plates were seeded with 300 l bacterial overnight cultures and were incubated for 2 days

This may be because NAD is an unstable molecule that does not survive long in a solution

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It is reported in [14] that the plasma adenosine concentration was significantly elevated on the average in autistic children compared to controls, though both populations showed very large standard deviations

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On the other hand, several drugs can deplete pyridoxine or heighten its requirement