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Before staining, slides were thawed to room temperature for 30 min and then fixed in 10% neutral buffered formalin for 20 min, rinsed in double-distilled water, followed by rinsing in 60% isopropanol before being placed in working ORO-isopropanol stain (Rowley Biochemical, H-503-1B) for 5 min

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These findings suggest that the local conformational change at S309 regulates RIPK1 activation through a mechanism independent of its intrinsic kinase activity

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33 Compared to tumor patients who did not receive antibiotic treatment before and after their first PD-1/PD-L1 treatment, the group receiving antibiotic treatment showed significantly shortened progression-free survival and overall survival

The proteins were then transferred onto nitrocellulose membranes via Western blotting