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cDNA was synthesized using the Revert Aid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) following the protocol, as given by the manufacturer

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Differences in sequencing depth between samples were accounted for in DESeq2 with median-of-ratio normalization

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The ginger extract, at doses of 200 and 300 mg/kg, enhanced the expression of IL-27 P28 and IL-27 EBI3