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Your body needs to keep its antioxidant supply "recharged." Once Glutathione neutralizes a free radical, it becomes "spent" or oxidized

After incubation, the batches were centrifuged at 4000 g for 25 minutes and the pellets were re-suspended in lysis buffer (200 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 2 mM benzamidine, 1 mM phenylmethylsulfonylfluoride and 0.5 g/l lysozyme, pH 7.8)