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[DOI] [PubMed] [Google Scholar] 895.Katsarou A., Moustakas I.I., Pyrina I., Lembessis P., Koutsilieris M., Chatzigeorgiou A

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Insulin tolerance tests were performed in 3-hourfasted mice by intraperitoneal injection of 1.4 mU insulin/kg body mass on chow diet or 2 mU insulin/kg body mass on HFD

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Cells were first stained with 50 M DCFDA or 10 M mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences