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For ELISA, liver tissues were sonicated in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100 and protease inhibitor cocktail (EDTA-free Complete Mini, Roche)

doi: 10.1038/s41392-024-02082-x 71 LiHLiuCLiRZhouLRanYYangQet al

961 p 5

Khavinson VKh, Lezhava TA, Monaselidze JR, Jokhadze TA, Dvalishvili NA, Bablishvili NK, Trofimova SV

littoralis appears to depend on GSTs present in multiple organ systems to protect itself against toxic ITCs, although these enzymes may also function in conjugating a range of other exogenous or endogenous metabolites

Patients should be able to verify their origin, manufacturer, and quality, said 10X Health's Carda