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Individual clones containing LacAnc100 and LaccID were inoculated into 50 l of SC minimal medium containing 6.7 g l 1 yeast nitrogen base (BD, 291940), 1.92 g l 1 yeast synthetic dropout medium supplement without uracil (Merck, Y1501), 20 g l 1 d-raffinose (Merck, R0250) and 25 g ml 1 chloramphenicol (Merck, C0378) in a sterile 96-well plate (Enzyscreen, CR1496c)

CDK9 and PP2A regulate RNA polymerase II transcription termination and coupled RNA maturation

S., & Horn, N

A requirement for beta-mercaptopyruvate in the in vitro thiolation of transfer ribonucleic acid

Thyroid 25 , 10691079 (2015)

This is because the soles are not really designed to last for such long periods, and in a few years, will likely end up crumbling away