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Samples were measured using high-resolution magic angle spinning (HR-MAS) NMR spectroscopy

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BPC-157 upregulates vascular endothelial growth factor (VEGF) receptors and activates endothelial nitric oxide synthase (eNOS), both critical for blood vessel formation and endothelial repair

To assess the robustness of the findings, additional sensitivity analyses were performed using spline-based ANCOVA with cubic regression splines to accommodate potential non-linear relationships between age and metabolite abundance