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The TCEs were diluted in 1xHBS-EP+ running buffer, and injected into all 6 flow cells as analytes at 37 C at a flow rate of 30 L/min with concentrations of 0.3125 nM, 0.625 nM, 1.25 nM, 2.5 nM, and 5 nM for AETX-R114 binding to soto-p 7 /A*03/11, 1.25 nM, 2.5 nM, 5 nM, 10 nM, and 20 nM for AETX-R114 binding to soto-p 7 /A*68 and AETX-R023 binding to soto-p 7 /A*03, and 5 nM, 10 nM, 20 nM, 40 nM, and 80 nM for AETX-R023 binding to soto-p 7 /A*11