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Higher serum selenium was associated with significantly lower odds (OR (95 % CI)) of autoimmune thyroiditis (047 (035, 065)), hypothyroidism (075 (063, 090)), subclinical hypothyroidism (068 (058, 093)) and enlarged thyroid (075 (059, 097)) ( Reference Wu, Rayman and Lv 75 )

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The purified RNA was then reverse transcribed into cDNA using the cDNA Reverse Transcription Kit (Thermo Fisher Scientific, USA)