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The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles Library 2: 4 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 3: 8 M 8-oxo-dGTP, 0.5 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library

The unmodified oligonucleotide elutes first (at ~ 1624 min), followed by the crude mixture of BP-DNA adducts dominated by BP- N 2 -dG adducts (collected at ~ 2635 min) and by a mixture of minor BP- N 6 -dA adducts (at ~ 3545 min), which are characterized by higher fluorescence intensities than the BP- N 2 -dG adducts

We have found that Sema4D-induced nuclear translocation of AR is dependent on Ran signalling and the GAP domain of PlexinB1, suggesting that PlexinB1/B2 contribute to ADT resistance through their function as RanGAPs

doi: 10.2307/4444260 46 SolainiGSgarbiGBaraccaA

Aumento da sntese proteica

doi: 10.1038/s41580-023-00648-1, 47 GaoZ.WangG.ChenY.YuanW.CaiJ.FengA.et al