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An equimolar concentration of Cu(II)acetate was added 2M in 50 mM Hepes, 50 mM NaCl, pH 7.4 and sample was incubated for up to 1 h

Human iPSCs were cultured in RPMI/B-27 (Thermo Fisher Scientific) with insulin and 6uM CHIR99021 (Stemgent) for 72 h at 37C 5% CO2 with daily media changes

BSO alone had minimal to no activity in six cell lines and had modest activity in the OPM-2, KMS-12-PE and MM.1S lines

Vegetarian:Yes