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Antioxidants also indirectly reduce oxidative damage by preventing the expression of enzymes responsible for generating ROS, such as NAD(P)H oxidase and xanthine oxidase, or by promoting the activity or expression of free radical scavengers

Sigma-Aldrich) was added to cells and incubated on ice under agitation for 12 min, the extraction buffer was then removed and stored on ice

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Conversely, Western-style diets, characterized by high saturated fat content and low nutritional diversity, promote gut dysbiosis and elevate cardiovascular risk markers [28], driving inflammatory and atherogenic processes [29]