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This stiffness can lead to shortness of breath and increased risk of lung infections

A suggested strategy is to use 2D cultures for rapid initial screening to identify relevant effects, and 3D models can be helpful for further studies of efficacy or cytotoxicity before using animal models [166]

After 2 15 min washes, Alexa 488-conjugated donkey anti-rabbit secondary antibody (Jackson Immunoresearch Europe Ltd., Cambridgeshire, United Kingdom

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(2002) first developed astrocyte-specific NF1 conditional knockout mice using Cre/loxP technology in which Cre was expressed in astrocytes by E14.5, and the astrocytes in these mice were NF1 null ( GFAPCre