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Cells were resuspended in annexin V binding buffer (BD Pharmingen) for staining and were analyzed using flow cytometry after 15 minutes of incubation at FL-1 and FL-3 channels

Cell culture All of the cell lines were obtained from Type Culture Collection of the Chinese Academy of Medical Science (Shanghai, China) and routinely tested for mycoplasma contamination

K.StemmeS

N-acetylcysteine as an adjuvant to clomiphene citrate for successful induction of ovulation in infertile patients with polycystic ovary syndrome