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A single colony was selected and cultured overnight at 37 C in 10 mL of terrific broth (TB) containing 100 g/mL ampicillin

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Nuclei were stained using 0.1 ug/ml DAPI (Thermo Fisher Scientific) for 10 minutes at room temperature, followed by PBS washing and mounting using Fluoromount-G (SouthernBiotech, Birmingham, AL, USA)