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When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature

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Berberine, found in plants like Coptis chinensis , has been shown to have antibacterial effects by intBerberineerfering with bacterial DNA replication and cell membrane integrity (Xiong et al., 2022)

Additionally, unique metabolites were identified between the model and bulb groups, implicating three primary metabolic pathways: amino acid metabolism, glucose metabolism, and the flavonoid pathway