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The assay is based on a luminescent bead-based system available from Perkin Elmer (Waltham, MA)

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Effect of Pretreatment with VPA and EMPA (1&3 mg/kg) on Immune-Expression of Apoptotic Marker (BAX) and Astrocyte Proliferation Marker (GFAP) in Hippocampal Sections in win- PTZ Induced Kindling in rats Results demonstrated that PTZ induced apoptosis in the hippocampal tissues, where the PTZ group showed diffuse brown immunostaining against BAX in the cytoplasm of CA3 neurons (Fig