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Elaidic acid potentiates extracellular ATP-induced apoptosis via the P2X7-ROS-ASK1-p38 axis in microglial cell lines

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To this end, cDNA sequences encoding the first 21, 30, 42, and 60 amino acids of GSH1 were translationally fused to a CrVenus reporter (Mackinder et al., 2016) and expression of the the GSH1 reporters (GSH1 n21 : Venus, GSH1 n30 :Venus, and GSH1 n42 :Venus) were verified by immunoblotting (Supplemental Figure S5A)

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