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Salen-based metallocompounds are protective in animal models of heart-ischemiareperfusion, amyotrophic lateral sclerosis, stroke, radiation-related lung fibrosis, and other diseases

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After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min

Serial dilutions of LUT, unloaded exosomes, and LUT-EX were added and allowed to incubate for 24 h at 37 C under 5% CO 2

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