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Description

Extracts from cells infected with the recombinant vaccinia virus are loaded onto Ni2+ nitriloacetic acid-agarose column and histidine-tagged proteins can be selectively eluted with imidazole-containing buffers

Magill, Brett W

[DOI] [PMC free article] [PubMed] [Google Scholar] 29.Yang H, Shen W, Liu W, Chen L, Zhang P, Xiao C, Chen X

Main body Two of the main players in the molecular and cellular response to OS are NRF2, the transcription nuclear factor erythroid 2-related factor 2, and its principal negative regulator, KEAP1, Kelch-like ECH (erythroid cell-derived protein with CNC homology)-associated protein 1

( 3.1.4 Nrf2/Slc7a11/GPX4 axis and myocardial injury In ischemia-reperfusion injury (IRI), a well-established model for myocardial injury, ozone pre-treatment mitigates myocardial ferroptosis by activating the Nrf2/solute carrier family 7 member 11 (Slc7a11)/GPX4 axis