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doi: 10.1557/s43578-021-00134-4

The development of KEAP1 and NRF2 knockout and conditional knockout GEMMs has significantly enhanced our understanding of KEAP1 and NRF2 function in both tumorigenesis and immunosurveillance (See Table 1 for references)

doi: 10.1002/jcb.27653 101 JollyMKPrecaBTTripathiSCJiaDGeorgeJTHanashSMet al

The parasite pellet (200 l) was mixed with 150 g of purified plasmid (pARL-1a+ hGrx1-roGFP2) in 400 l of cytomix and then electroporated at 0.310 kV and 950 F (Gene pulser, Bio-Rad) as described [34]

Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)