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In recent years, the rise of metabolomics has provided new ideas and methods for exploring the pathogenesis of COPD (67)

Peptide Vials (IGF-1 LR3, 1 mg each): 8 weeks 3 vials (~2.24 mg total needed) 12 weeks 4 vials (~3.64 mg total needed) 16 weeks 6 vials (~5.04 mg total needed) Insulin Syringes (U-100, 0.5 mL or 1 mL): Per week: 7 syringes (1/day) 8 weeks: 56 syringes 12 weeks: 84 syringes 16 weeks: 112 syringes Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for reconstitution

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Abnormal lipid metabolism Lipids are essential for the maintenance of cellular morphology and function, including biofilm composition, metabolism, energy storage, and signaling (Saleem et al., 2023)

RT-qPCR analysis Total RNA was extracted using TRI Reagent (Sigma-Aldrich)

The synergism of high-intensity intermittent exercise and every-other-day intermittent fasting regimen on energy metabolism adaptations includes hexokinase activity and mitochondrial efficiency